Improved DNA equilibrium binding affinity determinations of platinum(II) complexes using synchrotron radiation circular dichroism.

نویسندگان

  • Dale L Ang
  • Nykola C Jones
  • Frank Stootman
  • Bahman Ghadirian
  • Janice R Aldrich-Wright
چکیده

The binding affinity of a series of square planar platinum(II) compounds of the type [Pt(A(L))(I(L))](2+), where A(L) is 1,2-diaminoethane and I(L) are 1,10-phenanthroline (phen), 4-methyl-1,10-phenanthroline (4Mephen), 5-methyl-1,10-phenanthroline (5Mephen), 4,7-dimethyl-1,10-phenanthroline (47Me2phen), 5,6-dimethyl-1,10-phenanthroline (56Me2phen) or 3,4,7,8-tetramethyl-1,10-phenanthroline (3478Me4phen) has been reinvestigated using Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy. The additional peaks exhibited considerably greater intensity than those observed between 200 and 400 nm affording additional binding affinity determinations. In addition, the authors have reviewed the various mathematical approaches used to estimate equilibrium binding constants and thereby demonstrate that their mathematical approach, implemented with Wolfram Mathematica, has merit over other methods.

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عنوان ژورنال:
  • The Analyst

دوره 140 12  شماره 

صفحات  -

تاریخ انتشار 2015